Our IVD platform is based on q-PCR technology. Our lab team just got trained on d-PCR technology (Digital PCR). Eventhough d-PCR is still mainly used in Research, we are evaluating the opportunity to prepare future products on this technology and therefore wanted to make sure that our lab team completely masters it.
Q-PCR TECHNOLOGY STILL MASSIVELY USED BY HOSPITAL
Our IVD platform combines gene expression identification/quantification and algorithms and is materialized by products destined to hospitals which today are massively using q-PCR technology. This is the reason why we have adopted this technology so that there would be no barrier to entry or adoption of our product in the form of capital investment.
q-PCR (quantitative Polymerase Chain Reaction) is a molecular biology technique used to quantify the amount of a specific DNA or RNA sequence in a sample. It is an advanced form of the traditional PCR method that allows real-time monitoring and quantification of the amplified product during the reaction.
D-PCR IS EMERGING
Mainly used in research, digital PCR (d-PCR) is emerging as a complementary technology to real-time PCR for precise quantification of nucleic acids. It can offer a high level of sensitivity and robust performance against inhibitors. Digital PCR is used in a variety of research applications, providing unparalleled confidence in the quantification of rare targets, copy number variation (CNV), gene expression and much more.
HOW DOES D-PCR WORK ?
Digital PCR works by compartmentalizing a bulk PCR reaction into thousands of nanoliter-scale reactions, each containing zero, one, or a few DNA molecules. By counting positive reactions and applying Poisson statistics, absolute quantification of the sample is achieved. d-PCR overcomes common limitations of q-PCR, such as the need for calibration curves, low precision when measuring rare targets, and lack of sensitivity under high background conditions.
We thank our local representative from Qiagen for having organized and driven this training.